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 Forum index » Archive » Archive: Cloverfield (1-18-08) » Cloverfield: General / Updates
[SPEC]Extremophiles
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Red Walrus
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Joined: 24 Jul 2007
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[SPEC]Extremophiles
The key to everything? Origins of Slusho ingredient.

Just putting it all together.

An extremophile is an organism that thrives in and may even require physically or geochemically extreme conditions that are detrimental to the majority of life on Earth.

http://en.wikipedia.org/wiki/Extremophiles

I think one of the most important things in all the new site is the mention of extremophiles:

YOSHIDA MEDICAL RESEARCH
"YMR is a genetic research firm specializing in deep sea bioprospecting. YMR implements Tagruato's advanced exploration technology to study extremophiles found only in the deepest parts of our oceans. The understanding of such organisms that thrive in conditions of extreme pressure, temperature, and toxicity has boundless pharmaceutical and industrial promise. "

It also seems Bold Futura uses extremophiles:

"A machine made with Bold Futura parts works where others fall to pieces. A vehicle made with Bold Futura equipment will travel where others cannot reach. Our unique ability to function in areas of severe temperature or pressure can have desirable implications for our clients. "

Tagruato deep sea drilling seem to make use of Ganu's discovery:

"Our scientists and engineers have created drilling technology that can withstand degrees of extreme pressure, cold, and heat never before thought possible."

"Tagruato has long been developing vehicles and machinery that will operate at the same capacity as the bulldozers and cranes you pass on the freeway each day, but also function at the ocean's greatest depths."

Slusho also uses extremophiles.
I think Ganu's secret ingredient are extremophiles or their by-products.
This is why the ingredient must be kept cold:

From Vernon Macdood:
"something the Japanese call"kaitei no mitsu",or Seabed's Nectar ", shipped in dry ice by a company out of Honshu called Tagruato."

From the 1-18-08 recipe:
"Please make sure to keep this one cold!"
"Chill it until it becomes cold "
"and then chill "
"run under cold water until chilled."

From the Slusho History:
"Because the ingredient was discovered on the deep
ocean floor, under amazing pressure and in the most extreme cold, Ganu
knew he had to serve the ingredient in a near-frozen state to preserve its
freshness!!"


Cryptobiosis
The way that tardigrades have adapted to environmental stress is to reversibly suspend their metabolism and to effectively isolate themselves from the changes. This state is known as cryptobiosis and is a truly death-like state. Cessation of metabolism in other organisms is called death. Metabolism in tardigrades can lower to less than 0.01% of normal, or be entirely undetectable and the water content of the body may decrease to less than 1%. Tardigrades have been revived from this state after more than 100 years and shown signs of life! However, experiments have only shown cryptobiosis under natural conditions of 12 years but frozen they could theoretically survive for ever! The change in environmental conditions determine which cryptobiotic method—anhydrobiosis, cryobiosis, osmobiosis, and anoxybiosis—will occur.

Most studies of cryptobiosis have been done on anhydrobiosis, the form of cryptobiosis initiated by desiccation. Tardigrades living in a limno-terrestrial habitat, such as moss or lichens, require that they can survive long periods of dryness. Anhydrobiosis involves an almost complete loss of body water and the animal can stay in this state for an long period of time. Tun formation is an essential part of the process, which can be done repeatedly throughout the life cycle, even by adults, resulting in a body that is constricted and folded up. The limbs invaginate, the body contracts and becomes folded.The tun formation is an active process requiring metabolism and synthesis of a protective sugar Trehelose. After the tun is formed further desiccation can take place in 0 % relative humidity and the tardigrade can still survive. Revival typically takes a few hours from natural habitats but depends upon how long the tardigrade has been in the anhydrobiotic condition.

Cryobiosis is cryptobiosis which is initiated by a reduction in temperature to below 0 °C and involves the ordered freezing of water within the body cells . Osmobiosis is a form of cryptobiosis initiated by a decreased water potential due to increased solute concentration in the surrounding solution.A reduction of the % of oxygen initiates a suspended state in tardigrades, but is mostly not considered a form of cryptobiosis—tardigrades in this state remain extended, turgid, and immobile but it can only last for days before they die.

If the extremophile is not kept freezing cold it comes back to life.
(new ingredient Slusho!comes to life!!!!!!--- this is the only sentence in Slusho! history which has six of the mysterious exclamation points,6!s' = six words back)
http://forums.unfiction.com/forums/viewtopic.php?t=21175

One such extremophile living on the ocean bottom is the Tardigrade or water bear. Notice the three claws on it's arms. May be a candidate for the monster.(if size increases from a small fish to an enormous whale)

http://en.wikipedia.org/wiki/Water_bear
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjun00/mmbearp.html



I found a real life counterpart of YOSHIDA MEDICAL RESEARCH

http://www.funcrypta.de/www/en/

The strategy of FUNCRYPTA, therefore, is:

(i) to use a cultured tardigrade species, which will be used for all investigations as a model organism,
(ii) to link molecular, biochemical, and physiological data by bioinformatic techniques and modelling, and
(iii) to transfer the knowledge to the industrial sector

Also:
http://www.bio-pro.de/en/region/stern/magazin/02623/index.html



BEHAVIOR

A fast "runner," moving in a very characteristic way, and does not use its fourth pair of legs. Almost stands up, like a mini carnivore dinosaur, when it attacks prey.

FEEDING ECOLOGY AND DIET
Tardigrades... have a spear-like mouth part called a "stylet" that they use to pierce their prey and suck their juices as though through a straw. (Like drinking a Slusho! Smile )

Exclusively carnivorous,
http://www.answers.com/topic/milnesium-tardigradum?cat=technology

EDIT to add: "Slusho! is so Extreme" quote is added November 7Th to Happy Talk.

Edit - description - MikeyJ

PostPosted: Mon Oct 01, 2007 7:50 pm
Last edited by Red Walrus on Tue Nov 27, 2007 7:58 pm; edited 9 times in total
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AnubisWarrior
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Personally I doubt this is "the key to everything". But Tagruato's business with extremophiles may provide some nice background information, for example the monster could exhibit some traits related to extremophiles or the company could be working wit them when they disturb the monster.

I think this was a good connection and gives us a better idea of what this organization deals with. Smile
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PostPosted: Mon Oct 01, 2007 8:04 pm
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Red Walrus
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The monster being a water bear is just a secondary thought...the most important thing is how important the Extremophiles seem to be to the company and how they might relate to it's subsidiaries.

PostPosted: Mon Oct 01, 2007 8:08 pm
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AnubisWarrior
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Ah, I understand.
Yes, maybe there will be more nods to extremophiles later and it won't take us long to pick up on them, especially if it turns up on Slusho!
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PostPosted: Mon Oct 01, 2007 8:09 pm
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McPhearson
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Hm. Real interesting finds you have here. I wonder if we are looking at the very creature that could spawn the Cloverfield Creature.

Even if not, this is interesting information that could support the 'awakening' theory that has been spoken of, where the drilling rigs awake the frozen dormant creature into a rage.

PostPosted: Mon Oct 01, 2007 8:13 pm
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Red Walrus
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AnubisWarrior wrote:
Ah, I understand.
Yes, maybe there will be more nods to extremophiles later and it won't take us long to pick up on them, especially if it turns up on Slusho!


There may already be hints in the Slusho site:

Because the ingredient was discovered on the deep
ocean floor, under amazing pressure and in the most extreme cold, Ganu
knew he had to serve the ingredient in a near-frozen state to preserve its
freshness!!

The mention of freezing cold and cool all over the site.

The air, water, land and space themes where extremophiles can live.

PostPosted: Mon Oct 01, 2007 8:17 pm
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McPhearson
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See, the thing about that darn Slusho! site is, it's so darn cheery (almost to the point of eerie) that looking for clues can drive a man crazy.

PostPosted: Mon Oct 01, 2007 8:26 pm
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Red Walrus
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I don't think the parafun wax subsidairy has too much to do with the secret ingredient because it is actually made with oil, people are forgeting is tagruato's main revenue.

PostPosted: Tue Oct 02, 2007 9:19 am
Last edited by Red Walrus on Wed Nov 28, 2007 3:01 am; edited 1 time in total
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keeno_82uk
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sounds like you are onto something. I'll watch your progress with this.

Not sure about the bear though, it'd have to have grown significantly to pose a threat

The extremophiles i suspect will be in the consumables, not have anything to do with monster.

PostPosted: Tue Oct 02, 2007 9:32 am
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Red Walrus
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keeno_82uk wrote:
sounds like you are onto something. I'll watch your progress with this.

Not sure about the bear though, it'd have to have grown significantly to pose a threat

The extremophiles i suspect will be in the consumables, not have anything to do with monster.


Yeah I think I'm on target with the consumables being extremophiles, the monster is just spec. But It would be a kick ass monster, something that can survive in the harsest conditions even space, and it's got a reason to be pissed-- Tagruato is taking all it's kin and feeding it to humans. There is mention of genetic research on the extremophiles so that could explain it's growth or there was an old gigantic ancient one awakened(cause they can "hibernate" forever). Slusho is full of growth references though, so I suspect what even the monster ends up being, it will grow from Tagruato's meddling.

PostPosted: Tue Oct 02, 2007 10:08 am
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m0r1arty
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Nice Post

I went down a similar road of investigating just around the time that 'bottom sea crab' became 'nectar'. When I found out the water bear was only tiny though I was a bit disappointed. I didn't give it anywhere near as much consideration as you have.

Extremophiles are things that exist in harsh conditions. That makes me think they can't exist in what we consider to be normal conditions. There is a long drawn out prose/blog that goes 'I get up have breakfast...really long bit about all the cell type stuff...and I go to bed with my wife.'

In the really long bit about all the cell type stuff he goes on to talk about the very wide range of applications they are getting from all the cells. How they split them up lots and then see which ones do what. Not happy with just curing cancer, wanting to find out how it works etc. Kinda like science, only on fast forward.

Somewhere in amongst each is what creates/awakes the monster? Probably.

This could all be a big distraction though and in the end we find out Slusho! is made from Herrings way down past the Red Shift of the ocean.

-m0r

PostPosted: Tue Oct 02, 2007 11:17 am
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MrToasty
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The Paraffun bits that would be relevant to me are these:

Preparing specimens for histology?
Testing samples in infrared spectroscopy?
Moderating neutrons?

The first two for sure could tie in with examining flora or fauna from the ocean's depths.

PostPosted: Tue Oct 02, 2007 11:26 am
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Red Walrus
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Tardigrade eggs
Similar to the secret ingredient pic

Tardigrade eggs are similar to the secret ingredient pic in having lotus like petals. Kinda interesting.
WATER BEAR EGGS.jpg
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PostPosted: Tue Oct 02, 2007 11:40 am
Last edited by Red Walrus on Wed Oct 03, 2007 2:55 pm; edited 1 time in total
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dr_worm
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If there is a connection between the kaitei no mitsu and the monster, I don't see why the monster wouldn't also be an extremophile.

But I doubt eating water bears gives one delusions of grandeur, and so I'm inclined to think that the talk about extremophiles is only referencing the monster and not the kaitei no mitsu.

PostPosted: Tue Oct 02, 2007 6:23 pm
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pegassissy
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Cryobiosis is a form of cryptobiosis that takes place in reaction to decreased temperature. To initiate cryobiosis, the organism freezes all of the water within its cells. This allows the organism to endure the freezing temperatures until more hospitable conditions return. Studies have shown that the longer an organism remains in cryobiosis, the longer it takes for the organism to come out of cryobiosis. This is because the organism must use its own energy to come out of cryobiosis, and the longer it stays in cryobiosis the less energy it has.
Therefore, your theory could make perfect sence. the new drilling station opens in the mid atlantic ridge, and "it" takes a couple of months to wake up. Plus with the hot weater (63 in january?) it may take it a while to warm up.
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PostPosted: Tue Oct 02, 2007 6:25 pm
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