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 Forum index » Diversions » Perplex City Puzzle Cards » PXC: Silver Puzzle Cards
[S2] [Dice set] #232 Gel Together
Moderators: AnthraX101, bagsbee, BrianEnigma, cassandra, Giskard, lhall, Mikeyj, myf, poozle, RobMagus, xnbomb
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MikeyjModerator
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Joined: 18 Oct 2004
Posts: 1847
Location: London

I've not had a decent sit down with this one yet, but some half finished thoughts that might kick someone else off:

1. twixwolfs link above to the ligand database is really handy, you can also get extra information about each one by searching PDB (protein databank) I think it had some extra data on each of the ligands. I was wondering whether I could get some binary out of the charges on each molecule or that there was some sort of link with the molecular weight (there's everything from single ions to great big beasty molecules in the lists), but have not been able to get anything useful yet. (Plus I need t'internet in my house aaaaagh!)

2. I thought that I started to see a pattern that linked ligands to blood clotting pathways (and then Gel Together would make a nice bit of sense too) but I don't know enough about human biology (I abandoned boring old eukaryotes before university) to work it out.

3. The arrangement of the ligand abbreviations is like a diagrammatic form of an electrophoresis gel (some examples here. You have lanes, in our case three, and bands in each of the lanes, sometimes with gaps between them. I'm not sure whether it's significant or just to make it pretty and fit in with "Gel" again. Getting one gap at a time, except at the end, if you read down each lane gives you word spacing, and it seems to me that with each ligand representing a letter you could have a sensible length of sentence?

With respect to the phylogenetics, it's the animals that are bugging me and that's what lead you down that route isn't it AndyAndyO?...do the ligands form a question (in a Broadside kind of way) and the animals relate to the answer? Phylogenetically I don't think there is enough DNA sequence information available for each of the organisms shown in order to construct a phylogenetic tree. And what would we do with it if we got one?

off for a ponder Smile
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PostPosted: Tue Mar 27, 2007 10:01 am
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Shaph
Boot

Joined: 08 Mar 2007
Posts: 15
Location: Chicago, IL

Another link... possibly helpful?

I'll post a link here that I posted over on the Perplex City forum:

Spoiler (Rollover to View):
More Fun with Ligands!


I dunno if it'll help, but the site shows nearly all of the ligands and some of the proteins for which they are ligands.

Spoiler (Rollover to View):
One thing I did note was that a lot of the proteins are connected to escherichia coli, or E. Coli. There also seemed to be connections to rats, bovines, and humans as well.


Not sure if any of this will help, but if anything, it makes for great reading.
*shooting star over head* The More You Know!

PostPosted: Tue Mar 27, 2007 2:25 pm
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Mima
Decorated


Joined: 17 Feb 2005
Posts: 260

Tried
Spoiler (Rollover to View):
escherichia coli
for you, sorry but a no go, and I am no help other than that!

PostPosted: Wed Mar 28, 2007 7:43 am
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AndyAndyO
Veteran

Joined: 09 Aug 2006
Posts: 70

Mima wrote:
Tried
Spoiler (Rollover to View):
escherichia coli
for you, sorry but a no go, and I am no help other than that!


Unlikely effort, since the solve page asks for a one-word answer!

PostPosted: Wed Mar 28, 2007 11:05 am
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Mima
Decorated


Joined: 17 Feb 2005
Posts: 260

AndyAndyO wrote:
Mima wrote:
Tried
Spoiler (Rollover to View):
escherichia coli
for you, sorry but a no go, and I am no help other than that!


Unlikely effort, since the solve page asks for a one-word answer!


Note to self: Read solve page before putting in answer!!

PostPosted: Wed Mar 28, 2007 12:07 pm
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AndyAndyO
Veteran

Joined: 09 Aug 2006
Posts: 70

Updated wrong answers list.

Updated list of wrong answers....
Spoiler (Rollover to View):

fropig
frippo
tigon
zorse
liger
ligand
electrophoresis
genesis
morphogenesis
morphogens
progenitor
organogenesis
deoxyribonucleicacid
monkey
elephant
tretretretre
hybridomas
phylogenetics
phylogenetic
dolly
sheep
neurulation
amniotes
wolphin
marozi
pumapard
ceretin
keratin
evolution
cenancestor
insulin
cadherin
transgenic
transferrin
spine
vertebra
osteoporosis
genome
genetics
LUCA
Eukaryotes
Homeobox
Adam
Cloning
Bse
Minotaur
Leopon
Cow
zedonk


Someone must be getting close!

PostPosted: Thu Mar 29, 2007 2:43 pm
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Rand0m
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Joined: 14 Mar 2006
Posts: 271
Location: London

I'm determined not to let this one fall to guesswork without at least some sort of fight to solve it properly.

I'm working on the principle that this isn't a hardcore scientific puzzle where we have to identify how a specific compound or substance behaves using gel electrophoresis - it seems too abstract for that (compared with the scientific silvers in season one) and I can't believe it's a coincidence that we have plausible word breaks and substances grouped together by similar weights.

So that leaves us trying to somehow convert these compounds into letters.

*scratches head*

Edit: Just tried

Spoiler (Rollover to View):
Subracting units of 26 from the weights in each 'word' (from here) hoping that each one would eventually yield a nice set of numbers of 26 and under that would convert into workable English using 1=A, 2=B etc.


Didn't work.
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PostPosted: Thu Mar 29, 2007 7:28 pm
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MikeyjModerator
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Joined: 18 Oct 2004
Posts: 1847
Location: London

Rand0m wrote:
I'm determined not to let this one fall to guesswork without at least some sort of fight to solve it properly.

I'm working on the principle that this isn't a hardcore scientific puzzle where we have to identify how a specific compound or substance behaves using gel electrophoresis - it seems too abstract for that (compared with the scientific silvers in season one) and I can't believe it's a coincidence that we have plausible word breaks and substances grouped together by similar weights.


I agree, a large number of those compounds aren't suitable for gel electrophoresis. But I think that we may have to use some more science in order to resolve the relationships between things. I think I nice big table of all the ligands that we have with their various properties is in order. I'm on the case Smile
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PostPosted: Fri Mar 30, 2007 7:15 am
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Rand0m
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Joined: 14 Mar 2006
Posts: 271
Location: London

Mikeyj wrote:
I think I nice big table of all the ligands that we have with their various properties is in order. I'm on the case Smile


Didya see this linked off the official site forums here?
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PostPosted: Fri Mar 30, 2007 11:59 am
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MikeyjModerator
Unfictologist


Joined: 18 Oct 2004
Posts: 1847
Location: London

Thanks Rand0m (anyone else finding three forums hard work?), they seem to have got contacts at the EBI...I can't compete Rolling Eyes , but I'll upload my spreadsheet anyway here in case people want to shuffle things around. Currently they're in order L-R reading across the card. The clustering of molecular weights in each of the words is curious. Could that mean a different alphabet for each word?
Ligands.xls
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xls

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Filename  Ligands.xls 
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Irrelevant musings.

PostPosted: Fri Mar 30, 2007 12:23 pm
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Zapateria
Greenhorn

Joined: 31 Jul 2004
Posts: 7

It's a long shot but:

Spoiler (Rollover to View):
could the names of the missing ligands spell out the question? Someone mentioned what and name as possible first words. There is no WHA but there is a NAM-NAG-NAM (if that counts) and a _NA on the list of ligands linked to above.


Apologies if that is just nonsense Razz

PostPosted: Mon Apr 02, 2007 10:48 am
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Shaph
Boot

Joined: 08 Mar 2007
Posts: 15
Location: Chicago, IL

Spoiler (Rollover to View):
Your idea might be the right one; however, I've no clue how to figure out which ligands would go into the gaps. It might be a good avenue to explore for a bit to see what pops up, though!

My new wave of exploration will probably be exploring the proteins each ligand is associated with to see if any solid matches occur. If not, I might re-explore an old idea I had from the first page: build the ligands with my molecules sets and try and build something- for some reason my brain keeps returning to this card and thinking it's a blueprint to build something.


PostPosted: Mon Apr 02, 2007 11:46 am
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Zapateria
Greenhorn

Joined: 31 Jul 2004
Posts: 7

Warning: guesswork beyond this point

Spoiler (Rollover to View):
Working on the assumption that the names of the missing ligands spell out the question we need to answer, there are a couple of possible first and second words. Questions that would give a one word answer (not a name or a date) could start with, for example, NAME, WHAT or HOW.

WHAT is excluded as there are no ligands starting with WH. HOW is excluded because, although there is an HO ligand, none of the ligands starting with W end with the beginning of a plausible word such as 'DOes', 'CAn' etc.

This leaves NAME, with 'Name the', 'name a' or 'name one' being reasonable guesses for the start of the question leads to:

1st ligand:

NA: Sodium Ion

2nd ligand:

MET: Methionine

(N.B. MET also related to E.Coli)

No MEO or MEA

3rd ligand:

Plenty of ligands start with HE and it's virtually impossible to hazard any kind of guess for the next word, although the list of HE_ ligands will give possibilities for the first letter.

(Ligands can be looked up using http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/vctr/ligands_search.pl?template=tmplt33. Didn't want to post direct links as they show through the spoiler tags)

Hopefully someone will be able to add these ligands in to check/help develop some kind of pattern in the table.



PostPosted: Tue Apr 03, 2007 7:49 am
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Albedo
Boot

Joined: 18 Feb 2007
Posts: 34

To add to Zapateria's idea above:

Spoiler (Rollover to View):
At least two cards in this season which are coded messages decode to give phrases rather then properly worded questions, which then lead to the answers. In these cases the phrases begin with "a" or "an", so these should be considered as well, and perhaps as more likely than anything else.


PostPosted: Fri Apr 06, 2007 12:37 pm
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Shaph
Boot

Joined: 08 Mar 2007
Posts: 15
Location: Chicago, IL

I think I've given up on molecule building (can't get anything to work out to anything) so I decided to try a bit of analysis into turning the ligand abbreviations into code. I'm pretty sure I hit a dead end but I figured I'd share it anyways:

Spoiler (Rollover to View):
After learning that electrophoresis can be used in regards to DNA, I thought I might swap all A's, T's, C's, and G's to see if it might create new ligands. All but 7 did create new ligands. Going on that, I looked at total atom number, non-hydrogen atom numbers, etc. but still couldn't get anything that resembled english. Sad


If I get any new ideas I'll post them up.

PostPosted: Mon Apr 09, 2007 5:22 pm
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