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 Forum index » Archive » Archive: Cloverfield (1-18-08) » Cloverfield: General / Updates
A Day In The Life of a YMR Doctor
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m0r1arty
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 		A Day In The Life of a YMR Doctor
By Dr. Hiro Takahashi
Found here and posted here as a backup. I think this guy has loads of clues in here.

Please use this thread to pull apart anything you think should be looked into. Below is the quote from the site.

Quote:
Hello! In the morning I wake up and I go to work!

At work, samples excavated from the ocean floor are brought back to the lab and spread out on a Petri dish containing some 'media': Nutrients for growth that are combined with agar to form a solid surface! Bacteria that were present in the samples will then grow on the dish, forming a 'colony': Generally a small circle of billions of cells that all started from a single cell/spore in the sample! Each individual colony is then transferred to its own plate and grown so that you have a pure isolate: A pure strain of one bacterial type! Once we have those, they are transferred to a liquid media (also contains nutrients, but no agar, so it stays liquid) and grown in an incubator (keeps bacteria warm and shakes them to aerate the media). This is called liquid fermentation! Whatever chemical compounds the bacteria might produce are often secreted from the inside of the cells into the surrounding liquid media. Afterwards we extract these compounds from the liquid media using a resin that broadly absorbs most compounds that would be interesting! This resin is then washed with organic solvents which transfers all these interesting compounds from the resin into the organic solvents. Organic solvents are things like ethanol, or acetone (in nail polish remover), dichloromethane (in paint thinner), etc.! These solvent washes, which now contain the compounds produced by the bacteria during fermentation, can then be concentrated and this produces a "crude extract": It contains a whole lot of compounds that were either produced by the bacteria or were present in the liquid media to begin with!

This crude extract (we generate thousands of extracts each year) is then tested in a screen to see if it has any interesting bioactivity! For example, somebody might develop a screen to see if a compound kills cancer cells. A simplified method might involve something like putting cancer cells into a dish, adding this crude extract, and then observing whether or not the cancer cells survive. In a real setting this would be very high throughput...each "dish" would be a tiny 'well' (about the volume of a few drops of water) arrayed in a 'plate'...a rectangular piece of plastic that contains a grid of these wells (usually plates contain 96, 384, or 1536 wells). So then each well of each plate would contain a few drops of a suspension of cancer cells. We add a different extract to each well and see if cancer cells live or die! This way you can screen thousands of compounds very quickly. You could also do this to look for new antibiotics! Instead of putting cancer cells into each well, you'd put some sort of pathogenic bacteria that you want a new antibiotic against. In reality these screens often end up being a lot more complicated because people don't just want to kill cancer or bacteria cells outright (often these compounds would be toxic to regular human cells too), so they develop a screen that targets something really specific, like a particular protein involved in cancer pathways or something!

If one of these crude extracts turns up as a 'hit' on a screen, we then go about finding out what the specific compound is in the extract that's responsible for this interesting bioactivity! Remember that the crude extract contained everything the bacteria made, which can be hundreds to thousands of compounds. To do this, we perform a fractionation of the crude extract, in which these hundreds of compounds in a single extract are separated based on their chemical properties. This generates a series of fractions, so let's say I had an extract with a thousand compounds that I separated into 100 fractions. Now each fraction contains maybe 10 compounds. Each of these fractions is then rescreened, and hopefully we find that the bioactivity is in a particular fraction. Now we know the active compound is one of 10! We then repeat this fractionation process on these 10 compounds, rescreen them and find the active one, and then identify what that compound is. If it's a new compound, somebody would go about studying whether or not it could be a useful drug. So for example, using the screen, we might know that the compound kills pathogenic bacteria, but it wouldn't be a useful drug if it's too toxic to people, or gets broken down in the body too quickly, things like that! This might involve a "clinical trial": Trying the drug on real people after it's been through plenty of tests and development!

After work, I go home and dine with my wife Akemi! Then I go to bed!


-m0r

PostPosted: Tue Oct 02, 2007 12:14 pm
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wargasm
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 		Hmm... i dont see anything that looks like a clue in there. it just seems like a slightly dumbed down narration of the scientific process.

however, i have been wrong before

PostPosted: Tue Oct 02, 2007 12:51 pm
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kosmopol
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Joined: 27 Aug 2007
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 		OK, I see some clues, especially about "crude extract" and "interesting bioactivity" it evokes. The dangerous experiments as stereotypic beginning of horror movies.

Generally, I think, Slusho is just a side-effect. The really danger is coming from YOSHIDA MEDICAL RESEARCH. They drill in the deap sea and stir up a hornets' nest, they arouse with their drill systems the deap sea monsters.

I can understand the monsters, it's like your neighbor turns ACDC at 3:00 in the night. The drilling plattforms are also installed (perhaps it was already mentioned in this forum) everywhere. Especially interesting is Chuai station (Mid-Atlantic Ridge, Opening: September 2007). Perhaps there sleeps the monster, who knows?

PostPosted: Tue Oct 02, 2007 1:14 pm
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keeno_82uk
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 		searched for blogs that Hiro has made just out of the blue, but never came up with anything. There was however a japanese musician by the same name.

PostPosted: Tue Oct 02, 2007 1:17 pm
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Ecks51
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kosmopol wrote:
OK, I see some clues, especially about "crude extract" and "interesting bioactivity" it evokes. The dangerous experiments as stereotypic beginning of horror movies.


True, but this isn't a horror movie. Monster movies and horror movies are two different types of film. They may share a few parallels, but they're very different.
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PostPosted: Tue Oct 02, 2007 1:25 pm
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jojo
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 		We've seen the overuse of exclamation points in the Slusho history page. Same writer? Or is there some exclamation point-based clue?

PostPosted: Tue Oct 02, 2007 1:25 pm
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Red Walrus
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jojo wrote:
We've seen the overuse of exclamation points in the Slusho history page. Same writer? Or is there some exclamation point-based clue?


Maybe the exclamation points are to show how Happy! everyone is who drinks Slusho!!!!!

PostPosted: Tue Oct 02, 2007 1:30 pm
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Ecks51
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Red Walrus wrote:
jojo wrote:
We've seen the overuse of exclamation points in the Slusho history page. Same writer? Or is there some exclamation point-based clue?


Maybe the exclamation points are to show how Happy! everyone is who drinks Slusho!!!!!


That's what I was thinking, and then there's this...
Ganu Yoshida wrote:
In the past, the reputation of Tagruato has been sullied by reports of mistreating their employees. I have personally seen to it that no such claims will ever be made again.


Half-off Slusho! cards will do that to a person, I guess. Smile
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PostPosted: Tue Oct 02, 2007 1:35 pm
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brettoniasam
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 		This statement from the Good Doctor stood out a little:

Quote:
So for example, using the screen, we might know that the compound kills pathogenic bacteria, but it wouldn't be a useful drug if it's too toxic to people, or gets broken down in the body too quickly, things like that! This might involve a "clinical trial": Trying the drug on real people after it's been through plenty of tests and development!


What if such a hypothetical drug isn't approved by the FDA, or is taking a long time to get through the bureaucratic red tape? Would they use human beings as guinea pigs...???

Just wondering, because (like others) I'm starting to believe that Jamie's "bladder infection," rather than being some lame unfunny joke, is actually an indication that she's been exposed to a virus far more sinister...one that might have even been introduced to her by Teddy Hanssen (knowingly or unknowingly).

Just a thought. I like conspiracy theories. Razz

PostPosted: Tue Oct 02, 2007 2:21 pm
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Henrik
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 		Slusho! makes people happy... Confused - to be honest it sounds like a drug, and people get 'high' after drinking - and, more importent, addicted... To create an addiction means that Tagruato Corp. will profit from its junkie consumers due to the secret ingredient and peoples biochemical "need" for a fix.. and the future perspective will be that Tagruato Corp. will hold the world in its hands due to the widespread need for Slusho! (or need to get high, so to speak..)

I donīt know about the other products beeing developed...

PostPosted: Tue Oct 02, 2007 2:25 pm
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dr_worm
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 		So it could be that the entire thing is one gigantic acid trip by an entire city high on Japanese Icees. Laughing

PostPosted: Tue Oct 02, 2007 6:28 pm
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brettoniasam
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dr_worm wrote:
So it could be that the entire thing is one gigantic acid trip by an entire city high on Japanese Icees. Laughing


***BATMAN ALERT***
...Hey, remember in "Batman Begins," when Scarecrow dumped that "fear drug" in Gotham's water supply and everyone started hallucinating, seeing "monsters" everywhere...???

...good times. Very Happy

PostPosted: Tue Oct 02, 2007 7:50 pm
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dr_worm
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 		Batman Begins=Best movie ever.
The Dark Knight=Bester movie ever.

Heck yeah!

PostPosted: Wed Oct 03, 2007 1:34 am
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m0r1arty
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jojo wrote:
We've seen the overuse of exclamation points in the Slusho history page. Same writer? Or is there some exclamation point-based clue?


Happens sometimes to characters that don't translate. I sent emails from China (in English) that had lots of them!!! and others.

I like the fact he pretty much goes through a simplified version of how his day is. I just think there is too much there for there to be no clues.

The human trials might have been pushed ahead quickly, or even successful until they realise an out-of-context issue - such as the monster.

I don't know,, I was just hoping someone could give it the eagle eye and point out something I'm obviously missing.

-m0r

PostPosted: Wed Oct 03, 2007 3:42 pm
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